This webinar offers an in-depth exploration of advanced bioconjugation strategies. The program will feature:
Bioconjugation Fundamentals: A concise overview of covalently linking biomolecules to synthetic compounds.
Chemical Selectivity and Process Optimization: An analysis of targeted conjugation chemistries utilizing natural amino acid residues (e.g., amino, thiol, carboxyl groups) and an exploration of alternative strategies like glycoPEGylation. Emphasis will be placed on optimizing reaction parameters—including reagent ratios, pH, and temperature—to achieve high yields and desired specificity.
Conjugate Vaccine Development: A segment dedicated to the strategic conjugation of poorly immunogenic antigens to highly immunogenic carrier proteins, exemplified by peptide-CRM197 conjugates, to elicit robust immune responses.
Integrated Manufacturing Solutions: An introduction to Biosynth’s capabilities in bioconjugate process development, GMP-compliant manufacturing from microgram to multi-gram scales, and comprehensive analytical method development and characterization, including the synthesis of activated PEGs and functionalized amphiphilic polymers.
Carbohydrates – in the form of mono-, oligo- and polysaccharides and both in free from and linked to other biomolecules – are involved in a large variety of biological processes. Consequently, it is often necessary to conjugate carbohydrates chemically to other entities, e. g. for the diagnostics of such events, for drug targeting, for conjugate vaccine development or for the development of novel materials, to name only a few.
This section will cover some strategies to chemically conjugate carbohydrates and the challenges that come with them for instance conjugation via the reducing vs nonreducing terminus or conjugation involving total synthesis vs conjugation of carbohydrates from natural sources.
- Differentiate between various bioconjugation strategies, including those targeting natural amino acid residues and alternative methods like glycoPEGylation, and understand the critical parameters (reagent ratios, pH, temperature) for optimizing reaction efficiency and specificity.
- Why conjugate carbohydrates chemically? – Conjugation via reducing vs nonreducing terminus – Conjugation involving total synthesis vs conjugation of unprotected (native) oligosaccharides.
- Explain the principles of conjugate vaccine development, specifically how carbohydrates and poorly immunogenic antigens are strategically linked to carrier proteins to enhance immune responses, and recognize the unique challenges associated with carbohydrate conjugation.
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